Mini-monster Humane TNF-β (tumor-necrosefactor Beta) ELISA Kit

TNF-β

P01374

This kit recognizes Human TNF-β in samples. No significant cross-reactivity or interference between Human TNF-β and analogues was observed.

LT; LTA; TNFB; TNFSF1; TNF Superfamily, Member 1; Lymphotoxin Alpha

Sandwich-ELISA

ELISA

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TNF-β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNF-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNF-β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TNF-β. You can calculate the concentration of Human TNF-β in the samples by comparing the OD of the samples to the standard curve.

3 h 30 min

Tissue homogenates, cell lysates and other biological fluids

31.25-2000 pg/mL

Both intra-CV and inter-CV are < 10%.

80%-120%

18.75 pg/mL

Ice bag

2-8°C,12 months

Human

This ELISA kit applies to the in vitro quantitative determination of Human TNF-β concentrations in serum, plasma and other biological fluids.