Mini-monster Menselijk EFG (epidermale groeifactor) ELISA Kit

EGF

P01133

This kit recognizes Human EGF in samples. No significant cross-reactivity or interference between Human EGF and analogues was observed.

URG; HOMG4; Beta-Urogastrone

Sandwich-ELISA

ELISA

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human EGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human EGF. You can calculate the concentration of Human EGF in the samples by comparing the OD of the samples to the standard curve.

3 h 30 min

Tissue homogenates, cell lysates and other biological fluids

7.81-500 pg/mL

Both intra-CV and inter-CV are < 10%.

80%-120%

4.69 pg/mL

Ice bag

2-8°C,12 months

Human

This ELISA kit applies to the in vitro quantitative determination of Human EGF concentrations in serum, plasma and other biological fluids.