Mini monster muis PF4 (plateletfactor 4) ELISA Kit

PF4

Q9Z126

This kit recognizes PF4 in samples.No significant cross-reactivity or interference between PF4 and analogues was observed.

Platelet factor 4; PF-4; C-X-C motif chemokine 4; Cxcl4; Scyb4

Sandwich-ELISA

ELISA

Cell Biology; Immunology

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PF4 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PF4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PF4 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 41 nm. The OD value is proportional to the concentration of PF4 . You can calculate the concentration of PF4 in the samples by comparing the OD of the samples to the standard curve.

3 h 30 min

Serum, plasma and other biological fluids

15.63-1000 pg/mL

Both intra-CV and inter-CV are < 10%.

80%-120%

9.38 pg/mL

Ice bag

2-8°C,12 months

https://789.bio/eb/iT9Yc2SE

Microplate reader

Mouse

This ELISA kit applies to the in vitro quantitative determination of Mouse PF4 concentrations in serum, plasma and other biological fluids.