Mini-monsterrat CX3CL1 (chemokine C-X3-C-Motiefligand 1) ELISA Kit

CX3CL1

O55145

This kit recognizes CX3CL1 in samples.No significant cross-reactivity or interference between CX3CL1 and analogues was observed.

ABCD-3; C3Xkine; CXC3; CXC3C; NTN; NTT; SCYD1

Sandwich-ELISA

ELISA

Immunology; Neuroscience

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CX3CL1 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CX3CL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CX3CL1 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 51 nm. The OD value is proportional to the concentration of CX3CL1 . You can calculate the concentration of CX3CL1 in the samples by comparing the OD of the samples to the standard curve.

3 h 30 min

Serum, plasma and other biological fluids

3.12-200 ng/mL

Both intra-CV and inter-CV are < 10%.

80%-120%

1.88 ng/mL

Ice bag

2-8°C,12 months

https://789.bio/eb/rQzx2NzY

Microplate reader

Rat

This ELISA kit applies to the in vitro quantitative determination of Rat CX3CL1 concentrations in serum, plasma and other biological fluids.