Mini-monsterrat MIP-1α (Macrofage Inflammatory Protein 1 Alpha) ELISA Kit

MIP-1α

P50229

This kit recognizes MIP-1α in samples.No significant cross-reactivity or interference between MIP-1α and analogues was observed.

CCL3; LD78ALPHA; MIP-1-alpha; MIP1A; SCYA3; G0S19-1

Sandwich-ELISA

ELISA

Immunology; Neuroscience

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MIP-1α . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MIP-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MIP-1α , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 53 nm. The OD value is proportional to the concentration of MIP-1α . You can calculate the concentration of MIP-1α in the samples by comparing the OD of the samples to the standard curve.

3 h 30 min

Serum, plasma and other biological fluids

15.63-1000 pg/mL

Both intra-CV and inter-CV are < 10%.

80%-120%

9.38 pg/mL

Ice bag

2-8°C,12 months

https://789.bio/eb/9khUQbTF

Microplate reader

Rat

This ELISA kit applies to the in vitro quantitative determination of Rat MIP-1α concentrations in serum, plasma and other biological fluids.