Mini Sample Human CXCL1 (Growth Regulated Onogene Alpha) ELISA Kit

CXCL1

P09341

This kit recognizes CXCL1 in samples.No significant cross-reactivity or interference between CXCL1 and analogues was observed.

NAP3; GRO1; GRO-A; MGSA; MGSA-A; SCYB1; FSP; CINC-1

Sandwich-ELISA

ELISA

Immunology; Innate Immunity; Chemokines; Signal Transduction; Signaling Pathway; Neuroscience

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CXCL1 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CXCL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CXCL1 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 37 nm. The OD value is proportional to the concentration of CXCL1 . You can calculate the concentration of CXCL1 in the samples by comparing the OD of the samples to the standard curve.

3 h 30 min

Serum, plasma and other biological fluids

78.13-5000 pg/mL

Both intra-CV and inter-CV are < 10%.

80%-120%

46.88 pg/mL

Ice bag

2-8°C,12 months

https://789.bio/eb/9zJdqQTG

Microplate reader

Human

This ELISA kit applies to the in vitro quantitative determination of Human CXCL1 concentrations in serum, plasma and other biological fluids.