Mini Sample Human ESM1 (Endothelial Cell Specific Molecule 1) ELISA Kit

ESM1

Q9NQ30

This kit recognizes ESM1 in samples.No significant cross-reactivity or interference between ESM1 and analogues was observed.

Endocan; Endothelial cell-specific molecule 1

Sandwich-ELISA

ELISA

Tags and Cell Markers

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ESM1 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ESM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ESM1 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 31 nm. The OD value is proportional to the concentration of ESM1 . You can calculate the concentration of ESM1 in the samples by comparing the OD of the samples to the standard curve.

3 h 30 min

Serum, plasma and other biological fluids

15.63-1000 pg/mL

Both intra-CV and inter-CV are < 10%.

80%-120%

9.38 pg/mL

Ice bag

2-8°C,12 months

https://789.bio/eb/EAA8McIP

Microplate reader

Human

This ELISA kit applies to the in vitro quantitative determination of Human ESM1 concentrations in serum, plasma and other biological fluids.