Mini Sample Human GAL3 (Galectine 3) ELISA Kit

GAL3

P17931

This kit recognizes GAL3 in samples.No significant cross-reactivity or interference between GAL3 and analogues was observed.

LGALS3; CBP35; GALBP; GALIG; L31; LGALS2; MAC2; Lectin; Galactoside-Binding Soluble 3

Sandwich-ELISA

ELISA

Cell biology; Neuroscience

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GAL3 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GAL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GAL3 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 32 nm. The OD value is proportional to the concentration of GAL3 . You can calculate the concentration of GAL3 in the samples by comparing the OD of the samples to the standard curve.

3 h 30 min

Serum, plasma and other biological fluids

0.31-20 ng/mL

Both intra-CV and inter-CV are < 10%.

80%-120%

0.19 ng/mL

Ice bag

2-8°C,12 months

https://789.bio/eb/ramIgkMz

Microplate reader

Human

This ELISA kit applies to the in vitro quantitative determination of Human GAL3 concentrations in serum, plasma and other biological fluids.