Mini Sample Mouse CD23 (Receptor II voor de regio Fc van Immunoglobuline E) ELISA Kit

CD23

P20693

This kit recognizes CD23 in samples.No significant cross-reactivity or interference between CD23 and analogues was observed.

FCER2; BLAST-2; CD23A; CLEC4J; FCE2; IGEBF; Fc epsilon RII Recommended name:Low affinity immunoglobulin epsilon Fc receptor Alternative names:Fc-epsilon-RII Lymphocyte IgE receptor

Sandwich-ELISA

ELISA

Cell Biology

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CD23 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CD23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CD23 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 46 nm. The OD value is proportional to the concentration of CD23 . You can calculate the concentration of CD23 in the samples by comparing the OD of the samples to the standard curve.

3 h 30 min

Serum, plasma and other biological fluids

78.13-5000 pg/mL

Both intra-CV and inter-CV are < 10%.

80%-120%

46.88 pg/mL

Ice bag

2-8°C,12 months

https://789.bio/eb/XYvPckst

Microplate reader

Mouse

This ELISA kit applies to the in vitro quantitative determination of Mouse CD23 concentrations in serum, plasma and other biological fluids.