Mini Sample Mouse CD40 (Cluster of Differentiation 40) ELISA Kit

CD40

P27512

This kit recognizes CD40 in samples.No significant cross-reactivity or interference between CD40 and analogues was observed.

CD40; Bp50; CDW40; TNFRSF5; p50; CD40 (protein) ; CD40 molecule

Sandwich-ELISA

ELISA

Cell Biology; Immunology

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CD40 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CD40 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CD40 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 47 nm. The OD value is proportional to the concentration of CD40 . You can calculate the concentration of CD40 in the samples by comparing the OD of the samples to the standard curve.

3 h 30 min

Serum, plasma and other biological fluids

0.16-10 ng/mL

Both intra-CV and inter-CV are < 10%.

80%-120%

0.09 ng/mL

Ice bag

2-8°C,12 months

https://789.bio/eb/Xl54Iph4

Microplate reader

Mouse

This ELISA kit applies to the in vitro quantitative determination of Mouse CD40 concentrations in serum, plasma and other biological fluids.