Mini Sample Mouse CFH (complementfactor H) ELISA Kit

CFH

P06909

This kit recognizes CFH in samples.No significant cross-reactivity or interference between CFH and analogues was observed.

Hf1; Complement factor H; Protein beta-1-H

Sandwich-ELISA

ELISA

Immunology

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CFH . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CFH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CFH , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 40 nm. The OD value is proportional to the concentration of CFH . You can calculate the concentration of CFH in the samples by comparing the OD of the samples to the standard curve.

3 h 30 min

Serum, plasma and other biological fluids

1.56-100 ng/mL

Both intra-CV and inter-CV are < 10%.

80%-120%

0.94 ng/mL

Ice bag

2-8°C,12 months

https://789.bio/eb/eD9sg0Jf

Microplate reader

Mouse

This ELISA kit applies to the in vitro quantitative determination of Mouse CFH concentrations in serum, plasma and other biological fluids.