MIP-2 LeeporterTM Luciferase Reporter-RAW264.7 Cell Line

The MIP-2 LeeporterTM Luciferase Reporter cell line is a stably transfected RAW264.7 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the MIP-2 promoter. Macrophage inflammatory protein 2 (MIP-2) is a small cytokine that belongs to the C-X-C chemokine family and is also known as CXCL2. MIP-2 is one of the major proinflammatory cytokines, which is induced by innate immune receptors such TLRs and Nods, and also mediates LPS-induced osteoclastogenesis. The MIP-2 induction by Toll-like receptor 4 (TLR4) ligand, LPS, is shown in Figure 1.

Functional Assay

Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

Dry ice

Immediately upon receipt, store in liquid nitrogen.

Application: Monitor the MIP-2 induction activity. Screen for activators or inhibitors of the MIP-2 signaling pathway. Culture conditions: Cells should be grown at 37°C with 5% CO2 using DMEM medium (w/ L-Glutamine, 4.5g/L Glucose and Sodium Pyruvate) supplemented with 10% heat-inactivated FBS and 1% Pen/Strep, plus 3 μg/ml of Puromycin (Note: Puromycin can be omitted during the reporter cell assays) . It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a 37°C water-bath, transfer to a tube containing 10 ml of growth medium without Puromycin, spin down cells, resuspend cells in pre-warmed growth medium without Puromycin, transfer resuspended cells to T25 flask and culture in 37°C-CO2 incubator. Leave the T25 flask in the incubator for 1~2 days without disturbing or changing the medium until cells completely recover viability and become adherent. Once cells are over 90% adherent, remove growth medium and passage the cells through trypsinization and centrifugation. At first passage, switch to growth medium containing Puromycin. Cells should be split before they reach complete confluence. Note: RAW264.7 cells may not be detached well by trypsinization only. So you may need to use a cell scraper to harvest the trypsinized cells. To passage the cells, detach cells from culture vessel with Trypsin/EDTA, add complete growth medium and transfer to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cells suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly. To achieve satisfactory results, cells should not be passaged over 16 times. Functional validation: A. Response of MIP-2 LeeporterTM – RAW264.7 cells to lipopolysaccharide (LPS) . 1. Plate MIP-2 LeeporterTM – RAW264.7 cells into a white solid-bottom 96-well microplate in 100 μl of growth medium at 1 x 105 cells/well and incubate cells at 37°C in a CO2 incubator for 4-6 hours. 2. Stimulate cells with different concentrations of LPS and incubate cells at 37°C in a CO2 incubator for 16 hours. 3. Equilibrate the plate to room temperature for 10 minutes. 4. Add 50 μl of luciferase assay reagent (Abeomics, Cat #17-1101; Refer to the reagent datasheet for the detailed luciferase assay protocol) per well. 5. Read the plate in 1-5 minutes to measure luminescence using a microplate luminometer.