MIS12 cDNA

MIS12 is a component of the MIS12 complex, which is required for kinetochore formation during mitosis and normal chromosome alignment and segregation. The MIS12 complex consists of MIS12, DSN1, NSL1 and PMF-1. MIS12 is part of a network of complexes that provide microtubule attachment and generates pulling forces from depolymerization.

2510025F08Rik, hMis12, KNTC2AP, MTW1

17p13.2

Human

PATGen (puc19-derived cloning vector)

ATGTCTGTGGATCCAATGACCTACGAGGCCCAGTTCTTTGGCTTCACGCCACAAACGTGCATGCTTCGGATCTACATTGCATTTCAAGACTACCTATTTGAAGTGATGCAGGCCGTTGAACAGGTTATTCTGAAGAAGCTGGATGGCATCCCAGACTGTGACATTAGCCCAGTGCAGATTCGCAAATGCACAGAGAAGTTTCTTTGCTTCATGAAAGGACATTTTGATAACCTTTTTAGCAAAATGGAGCAACTGTTTTTGCAGCTGATTTTACGTATTCCCTCAAACATCTTGCTTCCTGAAGATAAATGTAAGGAGACACCTTATAGTGAGGAAGATTTTCAGCATCTCCAGAAAGAAATTGAACAGTTACAGGAGAAGTACAAGACTGAATTATGTACTAAGCAGGCCCTTCTTGCAGAATTAGAAGAGCAAAAAATTGTTCAGGCCAAACTCAAACAGACGTTGACTTTCTTTGATGAGCTTCATAATGTTGGCAGAGATCATGGGACTAGTGATTTTAGGGAGAGTTTAGTATCCCTGGTTCAGAACTCCAGAAAACTACAGAACATTAGAGACAATGTGGAAAAGGAATCGAAACGACTGAAAATATCTTAA

MIS12, 2510025F08Rik, hMis12, KNTC2AP, MTW1, MIS12, ATGD0332-10 µg, ATGD0332-20 µg, ATGD0332-50 µg, ATGD0332-100 µg, ATGD0332-250 µg, ATGD0332-500 µg, ATGD0332-1 mg, ATGD0332-10, ATGD0332-20, ATGD0332-50, ATGD0332-100, ATGD0332-250, ATGD0332-500, ATGD0332-1

Store the plasmid at -20C.

Lyophilized

Cell Cycle

609178

NP_076944.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_024039.2

618bp

This shuttle vector contains the complete ORF. It is inseted Nde I to Hind III. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.