Mitochondriale transcriptiefactor 1 / TFAM

MtTFA (mitochondrial transcription factor A), also known as mtTF1, TFAM, TCF6 (for transcription factor 6-like1), TCF6L2 and tsHMG, is a nuclear-encoded gene product that is imported into the mitochondria. mtTFA is required for many aspects of mitochondrial biogenesis including the replication and transcription of mitochondrial DNA (mtDNA) . In mouse, testis-specific mtTFA is missing the mitochondria targeting sequence and is present in the nucleus rather than the mitochondria. This form of mtTFA is located primarily in the nuclei of elongated spermatids and may be involved in the regulation of gene expression of the haploid male genome. During mouse and human spermatogenesis there is a reduction of mtTFA protein levels and a reduction in mtDNA copy number. These features may provide one of the mechanisms by which paternal mtDNA transmission is prevented. mtTFA has been associated with mitochondrial disorder in humans characterized by ocular myopathy, exercise intolerance and muscle wasting.

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

Q00059

Human

A portion of amino acids 1-200 from human Mitochondrial transcription factor 1 protein was used as the immunogen for the mtTFA antibody.

TFAM/9713

IHC-P, WB

Protein A/G affinity

Purified

This mtTFA antibody is available for research use only.

Aliquot the mtTFA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the mtTFA antibody should be determined by the researcher.

Cytoplasm (Mitochondria)

Western blot testing of human HEK293T cell lysate with mtTFA antibody (clone TFAM/9713) . Expected molecular weight: 24-29 kDa.