MMP 8 Recombinant eiwit

Source : Escherichia Coli. Matrix Metalloproteinase-8 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 75 kDa.The MMP-8 is purified by proprietary chromatographic techniques. Full-length recombinant human neutrophil pro-collagenase (MMP-8), latent form.Matrix metalloproteinase 8 (MMP-8), or neutrophil collagenase, degrades interstitial collagens, acting preferentially on collagen type I. Increased full-length MMP-8 protein was associated with infiltration into the skin of neutrophils, which are the major cell type that expresses MMP-8.MMP-8 is synthesized and stored in specific granules in neutrophil leukocytes. MMP-8 activity is therefore regulated by factors such as surface-bound ligands (IgG or complement components) that release it through degranulation.Once released and activated through proteolytic or oxidative mechanisms, MMP-8 plays a major role in the connective tissue turnover that accompanies inflammatory processes.

Neutrophil collagenase||EC 3.4.24.34||Matrix metalloproteinase-8||MMP-8||PMNL collagenase||PMNL-CL||HNC||CLG1.

Greater than 90% as determined by SDS-PAGE.

The MMP-8 protein solution (100 units/ml) in 0.05M Tris-HCl buffer, pH 7.6, 0.2M NaCl, 5mM CaCl2, 0.0025% NaN3 and 0.1% BSA.

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

100 units/ml after activation with APMA by solution assay method.One unit of collagenolytic activity is defined as the cleavage of 1μg of collagen per minute by the solution method.