Monoklonale antilichamen tegen cytokeratine 5/8 (kloon: SPM268)

It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa) . Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.

KRT5

KRT5

3852

P13647

Human, Mouse, Rat, Hamster

Cytoskeletal prepaRation from HeLa cells

Cytokeratin 5/8

SPM268

FACS, IF, WB, IHC

Affinity Chromatography

Purified

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Western Blot (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 15 min at RT)

Mouse IgG1, kappa