Mouse IL-1 Alpha (Interleukin-1 Alpha) Pre-Coated ELISA Kit

This assay employs an antibody specific for Anti-mouse IL-1a coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1a present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-1a antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1a bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Mouse

ELISA

1 x 96 well Format (96 Tests)

Please refer to the Manual

https://www.abeomics.com/images/90-2254/Mouse%20IL-1Alpha_Manual.pdf

This immunoassay kit allows for the in vitro quantitative determination of Mouse IL-1a concentrations in serum, plasma and other biological fluids. Detection Range: 15.6- 1000 pg/ml Sensitivity: < 9.4 pg / ml