Mouse Monoclonal Antibody to CBP Tag (Clone: 1D11E5)

CBP affinity tag is a useful tag in protein study which can be added to the N or C terminus of proteins of interest through DNA recombinant technology. The tag is derived from muscle myosin light-chain kinase. The tag comprises 26 amino acid residues with the molecular weight of 4 kDa and sequence of KRRWKKNFIAVSAANRFKKISSSGAL. CBP tag has the relatively high affinity for calmodulin (CaM) . CBP-tagged proteins can be purified from crude cell extracts through CaM affinity resin. CBP Tag antibody is a useful tool in analysis of CBP-tagged proteins. CBP Tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes harvested from mouse immunized with CBP tag peptide conjugated to KLH.

CBP tag peptide conjugated to KLH

1D11E5

ELISA

Protein A chromatography

Purified

0.5 mg/mL, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Western blot: 1-2 μg/mL. Immunoprecipitation: 1-2 μg/mL. ELISA: 0.001-0.002 μg/mL.

Mouse IgG2b, Kappa