MTHFS cDNA

MTHFS an enzyme that catalyzes the conversion of 5-formyltetrahydrofolate to 5, 10-methenyltetrahydrofolate, a precursor of reduced folates involved in 1-carbon metabolism. An increased activity of the encoded protein can result in an increased folate turnover rate and folate depletion. Three transcript variants encoding two different isoforms have been found for MTHFS.

HsT19268

15q25.1

Human

PATGen (puc19-derived cloning vector)

ATGGCGGCGGCAGCGGTGAGCAGCGCCAAGCGGAGCCTGCGGGGAGAGCTGAAGCAGCGTCTGCGGGCGATGAGTGCCGAGGAGCGGCTACGCCAGTCCCGCGTACTGAGCCAGAAGGTGATTGCCCACAGTGAGTATCAAAAGTCCAAAAGAATTTCCATCTTTCTGAGCATGCAAGATGAAATTGAGACAGAAGAGATCATCAAGGACATTTTCCAACGAGGCAAAATCTGCTTCATCCCTCGGTACCGGTTCCAGAGCAATCACATGGATATGGTGAGAATAGAATCACCAGAGGAAATTTCTTTACTTCCCAAAACATCCTGGAATATCCCTCAGCCTGGTGAGGGTGATGTTCGGGAGGAGGCCTTGTCCACAGGGGGACTTGATCTCATCTTCATGCCAGGCCTTGGGTTTGACAAACATGGCAACCGACTGGGGAGGGGCAAGGGCTACTATGATGCCTATCTGAAGCGCTGTTTGCAGCATCAGGAAGTGAAGCCCTACACCCTGGCGTTGGCTTTCAAAGAACAGATTTGCCTCCAGGTCCCAGTGAATGAAAACGACATGAAGGTAGATGAAGTCCTTTACGAAGACTCGTCAACAGCTTAA

MTHFS, HsT19268, MTHFS, ATGD0265-10 µg, ATGD0265-20 µg, ATGD0265-50 µg, ATGD0265-100 µg, ATGD0265-250 µg, ATGD0265-500 µg, ATGD0265-1 mg, ATGD0265-10, ATGD0265-20, ATGD0265-50, ATGD0265-100, ATGD0265-250, ATGD0265-500, ATGD0265-1

Store the plasmid at -20C.

Lyophilized

Metabolism

604197

NP_006432.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_006441.3

612bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.