MUC-1 Antilichaam

MAb VU-2G7 reacts with MUC1/Mucin-1/Epithelial Marker Antigen/EMA, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The dominant epitope of VU-2G7 has not been established with epitope fingerprinting. The extra cellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion.

ELISA: order BSA/sodium azide-free format for coating

P15941

Human

A synthetic glycosylated MUC1 60mer tandem repeat NH2- (HGVTSAPDT (GalNAc) RPAPGSTAPPAHG) 3- COOH was used as the immunogen for the MUC-1 antibody.

VU-2G7

ELISA

Protein G affinity chromatography

Purified

This MUC-1 antibody is available for research use only.

Store the MUC-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Optimal dilution of the MUC-1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min

Cytoplasmic and cell surface

SDS-PAGE Analysis of Purified, BSA-Free MUC-1 Antibody (clone VU-2G7) . Confirmation of Integrity and Purity of the Antibody.