MUC5AC Antilichaam

This MAb recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC) . Its epitope is located in the C-terminal cysteine rich part of the peptide core of MUC5AC. Its epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.

Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 minutes at RT

P98088

Human, Mouse, Rat

An M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le (a-b) patient was used as the immunogen for the biotinylated MUC5AC antibody.

45M1

IHC-P

Protein G affinity chromatography

Biotin Conjugate

This biotinylated MUC5AC antibody is available for research use only.

Store the biotinylated MUC5AC antibody at 2-8°C (up to one month) or aliquot and store at -20°C (longer term) .

0.2 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the biotinylated MUC5AC antibody should be determined by the researcher.

Cytoplasmic

IHC staining of FFPE human gastric carcinoma with biotinylated MUC5AC antibody (clone 45M1) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.