Mug cDNA

G/u mismatch-specific DNA glycosylase, xanthine DNA glycosylase, also known as mug, belongs to the TDG/mug DNA glycosylase family. It has been proposed that the Mug protein excises 3, N4-ethenocytosine and removes the uracil base from mismatches in the order of u:G>u:A, although the biological role remains unclear.

Dug, ECK3058, JW3040, ygjF

E.coli

PATGen (puc19-derived cloning vector)

ATGGTTGAGGATATTTTGGCTCCAGGGTTACGGGTCGTGTTTTGCGGTATCAACCCTGGGCTTTCATCCGCCGGGACTGGTTTTCCCTTTGCTCATCCGGCAAATCGCTTCTGGAAGGTGATATATCAGGCCGGGTTTACCGACCGTCAGTTGAAGCCGCAGGAGGCACAGCATCTGCTGGATTATCGTTGTGGCGTCACCAAACTGGTAGACCGTCCAACGGTGCAAGCCAATGAAGTTTCAAAGCAGGAGCTACACGCAGGCGGGCGTAAGCTGATTGAAAAAATTGAAGATTATCAGCCGCAGGCGTTGGCGATTCTGGGCAAACAAGCATATGAACAGGGATTCAGCCAGCGCGGTGCACAGTGGGGGAAACAAACGCTCACCATTGGTTCGACGCAGATTTGGGTGCTGCCAAATCCCAGCGGTTTAAGTCGCGTTTCACTGGAGAAACTGGTTGAAGCGTATCGCGAGCTGGACCAGGCGCTGGTAGTGCGTGGGCGATAA

Mug, dug, ECK3058, JW3040, ygjF, mug, ATGD0438-10 µg, ATGD0438-20 µg, ATGD0438-50 µg, ATGD0438-100 µg, ATGD0438-250 µg, ATGD0438-500 µg, ATGD0438-1 mg, ATGD0438-10, ATGD0438-20, ATGD0438-50, ATGD0438-100, ATGD0438-250, ATGD0438-500, ATGD0438-1

Store the plasmid at -20C.

Lyophilized

Enzymes & Proteases

NP_417540.1

E.coli

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NC_000913.2

507bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.