Muty cDNA

Adenine DNA glycosylase, also known as mutY, is an adenine DNA glycosylase active on DNA substrates containing A/G, A/8-oxoG, or A/C mismatches and also has a weak guanine glycosylase activity on G/8-oxoG-containing DNA. mutY is crucial for the avoidance of mutations resulting from oxidative DNA damage.

E.coli

PATGen (puc19-derived cloning vector)

ATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGGAAAACGCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTGTGGACCGGGCTTGGCTATTACGCCCGCGCGCGCAACCTGCATAAAGCGGCACAACAAGTGGCGACCTTACACGGCGGTAAATTCCCGGAAACCTTTGAAGAAGTCGCGGCGTTACCGGGCGTCGGGCGTTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGGCCTGGGAAAAAAGAGGTCGAGAATAAACTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGCGCGATGATTTGTACGCGCTCTAAGCCGAAATGTTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGTCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGCCAGAGCGCACCGGCTACTTTTTGCTGTTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGACAGATTTCTGCCGATAACCTGACGCAGCTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTGGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAG

MutY, mutY, ATGD0439-10 µg, ATGD0439-20 µg, ATGD0439-50 µg, ATGD0439-100 µg, ATGD0439-250 µg, ATGD0439-500 µg, ATGD0439-1 mg, ATGD0439-10, ATGD0439-20, ATGD0439-50, ATGD0439-100, ATGD0439-250, ATGD0439-500, ATGD0439-1

Store the plasmid at -20C.

Lyophilized

Enzymes & Proteases

YP_002413998.1

E.coli

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NC_000913.2

1053bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.