MYC-tag antilichaam

Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The Myc tag corresponds to amino acid residues (EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino-or carboxy-terminus of targeted proteins.

Myc|MYC-tag|EQKLISEEDL

Recombinant protein

MYC-tag

MYC-tag

Protein A+G purification

WB: 1:1000-1:5000; IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

≥95% as determined by SDS-PAGE

Liquid

4°C with ice bag

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

6C4

ELISA, WB, IP

Peptide