MYD88 Antilichaam

MYD88, myeloid differentiation primary response 88, was identified as an innate immune signal transduction adaptor involved in the Toll-like receptor (TLR) and interleukin-1 (IL-1) signaling pathway (1,2,3) and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 functions as an adaptor protein for TLRs and IL-1 receptors, which stimulates IRAKs, IRF7 and TRAF6, leading to NF-κB activation, cytokine secretion and inflammatory response (2, 4,5,6) . Nuclear factor-kappa-B activation modulates multiple genes regulating the body's immune reactions and inflammatory responses. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R-mediated NF-κB activation (4,5) . MyD88 is also employed as a regulator molecule by IL-18 receptor. Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs TLR2 and TLR4 as the signaling receptors (7,8) . MyD88 gene is expressed in many tissues.

4615

U70451

AAB49967.1

Human, Mouse, Rat

Unconjugated

Primary Antibodies

KO validation (Figure 1) : Anti-MYD88 antibody (2127) specificity was further verified by MYD88 specific knockout. MyD88 signal was not detected in MyD88 knockout HeLa cells as compared to that in control wild type cells. KO validation2127 Independent Antibody Validation in Cell lines (Figure 3) shows similar MYD88 expression profile in both human and mouse cell lines detected by two independent anti-MYD88 antibodies that recognize different epitopes, 2125 against internal domain and 2127 against the C-terminus domain. MYD88 proteins are detected in all the tested cell lines at different expression levels by the two independent antibodies. Additionally, Figure 2 shows the mouse MYD88 protein in NIH/3T3 cells migrates slightly faster than human isoform 1 detected by both MYD88 antibodies (2125 and 2127), which is well correlated with their calculated molecular masses (33.8 kDa vs 35.4 kDa) . Independent Antibody Validation in Cell lines2125 2127 Independent Antibody Validation in Human Tissues (Figure 4) shows similar MYD88 expression profile in human tissues detected by two independent anti-MYD88 antibodies (2125 and 2127) . MYD88 proteins are detected by the two independent antibodies in liver, kidney, lung, thymus, colon, bladder and breast of human tissues at different expression levels, but not in heart, brain, skin and pancreas. Independent Antibody Validation in Human Tissues Animal Species Reactivity (Figure 5) : Anti-MYD88 antibodies (2125 and 2127) can detect the expression of MYD88 protein in the liver and spleen of all tissues and mouse heart, but not in human heart. Additionally, Figure 4 also shows MYD88 protein detected by both MYD88 antibodies (2125 and 2127) in human liver and Daudi cells migrates slightly slower than that in the tissues of mouse and rat, which is well correlated with their calculated molecular masses (35.4 kDa vs 33.8 kDa and 33.9kD) . Animal Species Reactivity21252127

Signal Transduction, Inflammasomes

MYD88 Antibody is affinity chromatography purified via peptide column.

Cat. No. 1202 - A431 Cell Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Pig: (94%), Sheep: (82%), Bovine: (82%), Chicken: (82%)

MYD88 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

MYD88 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

Human MYD88 has 7 isoforms, including isoform 1 (317aa, 35.4kD), isoform 2 (296aa, 33.2 kD), isoform 3 (251aa, 28.3kD), isoform 4 (191aa, 20.8kD), isoform 5 (146aa, 15.8kD), isoform 6 (275aa, 31.5kD), and isoform 7 (304aa, 34.1kD) . This antibody detects human isoform 1,2,3,6,7, but not isoform 4,5. Mouse MYD88 has two isoforms, including isoform 1 (296aa, 33.8kD) and isoform 2 (250aa, 28.7kD) . Rat MYD88 has only one isoform identified so far (296aa, 33.9kD) .

MYD88

Cat. No. 1203 - A549 Cell Lysate

Cat. No. 1204 - K562 Cell Lysate

Cat. No. 1211 - HepG2 Cell Lysate

Cat. No. 1282 - NIH/3T3 Cell Lysate

Myeloid differentiation primary response gene (88)

Homo sapiens

Medzhitov et al. Mol. Cell. 1998;2:253-8

Kawai et al. Nat. Immunol. 2004;5:1061-8

Semaan et al. J. Immunol. 2008;180:3485-91

Muzio et al. Science 1997;278:1612-5

Yamamoto et al. Mol. Immunol. 2014;58:66-76

Ohnishi et al. Proc. Natl. Acad. Sci. U.S.A. 2009;106:10260-5

Adachi et al. Immunity 1998;9:143-50

Kawai et al. Immunity 1999;11:115-22

21233312

28674182

17576821

15361868

15349893

16413925

18583567

17485511

12538665

25761602

23962979

17905570

19638630

23844229

20459723

15961329

21902690

19266568

12646618

26671458

MYD88 Antibody: MYD88D

ELISA, WB, IF, IP

1763090

Liquid