NAPA cDNA

NAPA is one of the SNAP (Soluble NSF Attachment Protein) family. SNAPs, acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. This protein mediates the binding of NSF and thus the formation of a 20S fusion particle. It is expressed in all mammalian tissues.

N-ethylmaleimide-sensitive factor attachment protein alpha, NSF attachment protein alpha, SNAPA, SNAP-alpha

19q13.33

Human

PATGen (puc19-derived cloning vector)

ATGGACAATT CCGGGAAGGA AGCGGAGGCG ATGGCGCTGT TGGCCGAGGC GGAGCGCAAA GTGAAGAACT CGCAGTCCTT CTTCTCTGGC CTCTTTGGAG GCTCATCCAA AATAGAGGAA GCATGCGAAA TCTACGCCAG AGCAGCAAAC ATGTTCAAAA TGGCCAAAAA CTGGAGTGCT GCTGGAAACG CGTTCTGCCA GGCTGCACAG CTGCACCTGC AGCTCCAGAG CAAGCACGAC GCAGCCACCT GCTTTGTGGA CGCTGGCAAC GCATTCAAGA AAGCCGACCC CCAAGAGGCC ATTAACTGTT TGATGCGAGC AATCGAGATC TACACAGACA TGGGCCGATT CACGATTGCG GCCAAGCACC ACATCTCCAT TGCTGAGATC TATGAGACAG AGTTGGTGGA CATCGAGAAG GCCATTGCCC ACTACGAGCA GTCTGCAGAC TACTACAAAG GCGAGGAGTC CAACAGCTCA GCCAACAAGT GTCTGCTGAA GGTGGCTGGT TACGCTGCGC TGCTGGAGCA GTATCAGAAG GCCATTGACA TCTACGAACA GGTGGGGACC AATGCCATGG ACAGCCCCCT CCTCAAGTAC AGCGCCAAAG ACTACTTCTT CAAGGCGGCC CTCTGCCACT TCTGCATCGA CATGCTCAAC GCCAAGCTGG CTGTCCAAAA GTATGAGGAG CTGTTCCCAG CTTTCTCTGA TTCCCGGGAA TGCAAGTTGA TGAAAAAATT GCTAGAGGCC CACGAGGAGC AGAATGTGGA CAGCTACACC GAGTCGGTGA AGGAATACGA CTCCATCTCC CGGCTGGACC AGTGGCTCAC CACCATGCTG CTGCGCATCA AGAAGACCAT CCAGGGCGAT GAGGAGGACC TGCGCTAA

SNAP-alpha, SNAPA, SNAP alpha, NSF attachment protein alpha, N-ethylmaleimide-sensitive factor attachment protein alpha, N-ethylmaleimide sensitive factor attachment protein alpha, N ethylmaleimide sensitive factor attachment protein alpha, ATGD0071-10 µg, ATGD0071-20 µg, ATGD0071-50 µg, ATGD0071-100 µg, ATGD0071-250 µg, ATGD0071-500 µg, ATGD0071-1 mg, ATGD0071-010, ATGD0071-020, ATGD0071-050, ATGD0071-100, ATGD0071-250, ATGD0071-500, ATGD0071-01M

Store the plasmid at -20C.

Lyophilized

Neuroscience

603215

NP_003818.2

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_003827.3

888bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.