NCF1 Antilichaam / Neutrofiel cytosolische factor 1 / p47phox

ELISA: 0.1-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Western Blot: 0.25-0.5 µg/mL

P14598

Human, Mouse, Rat

E.coli-derived human p47 phox/NCF1 recombinant protein (Position: Q22-R384) was used as the immunogen for the NCF1 antibody.

ELISA, FACS, IF, IHC, WB

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This NCF1 antibody is available for research use only.

After reconstitution, the NCF1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the NCF1 antibody should be determined by the researcher.

Cytoplasmic

Immunohistochemical staining of NCF1 using anti- NCF1 antibody. NCF1 was detected in a paraffin-embedded section of rat lymph tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti- NCF1 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.