NCF4 Mouse Monoclonal Antibody [Kloon ID: LBI4B5]

The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI (3) kinase, which suggests its role in PI (3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Carrier-free (BSA/glycerol-free) NCF4 mouse monoclonal antibody, clone LBI4B5

NCF; P40PHOX; SH3PXD4

Homo sapiens neutrophil cytosolic factor 4 (NCF4), transcript variant 1, mRNA.

4689

Q15080

Unconjugated

Leukocyte transendothelial migration

Leukocyte transendothelial migration

WB 1:2000, IHC 1:150

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

NCF4

https://www.ncbi.nlm.nih.gov/gene/?term=4689

https://www.uniprot.org/uniprot/Q15080