NCL Antilichaam / Nucleoline

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. [UniProt]

Western blot: 1:500-1:2000

P19338

Human

A synthetic peptide specific to human Nucleolin protein was used as the immunogen for the NCL antibody.

29N05

WB

Affinity chromatography

Purified

This NCL antibody is available for research use only.

Store the NCL antibody at -20°C.

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Optimal dilution of the NCL antibody should be determined by the researcher.

Western blot testing of human HeLa cell lysate with NCL antibody. Predicted molecular weight ~77 kDa, commonly observed at 100~110 kDa.