NDUFA2 cDNA

NDUFA2 is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex 1), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane, and may be involved in regulating complex I activity or its assembly via assistance in redox processes. Mutations in this gene are associated with Leigh syndrome, an early-onset progressive neurodegenerative disorder. Alternative splicing results in multiple transcript variants.

B8, CD14, CIB8

5q31.2

Human

PATGen (puc19-derived cloning vector)

ATGGCGGCGGCCGCAGCAAGTCGAGGAGTCGGGGCAAAGCTGGGCCTGCGTGAGATTCGCATCCACTTATGTCAGCGCTCGCCCGGCAGCCAGGGCGTCAGGGACTTCATTGAGAAACGCTACGTGGAGCTGAAGAAGGCGAATCCCGACCTACCCATCCTAATCCGCGAATGCTCCGATGTGCAGCCCAAGCTCTGGGCCCGCTACGCATTTGGCCAAGAGACGAATGTCCCTTTGAACAACTTCAGTGCTGATCAGGTAACCAGAGCCCTGGAGAACGTTCTAAGTGGTAAAGCCTGA

NDUFA2, B8, CD14, CIB8, NDUFA2, ATGD0284-10 µg, ATGD0284-20 µg, ATGD0284-50 µg, ATGD0284-100 µg, ATGD0284-250 µg, ATGD0284-500 µg, ATGD0284-1 mg, ATGD0284-10, ATGD0284-20, ATGD0284-50, ATGD0284-100, ATGD0284-250, ATGD0284-500, ATGD0284-1

Store the plasmid at -20C.

Lyophilized

Metabolism

602137

NP_002479.

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_002488.4

300bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.