NDUFAB1 Antilichaam / NADH dehydrogenase ubichinon 1 alfa subcomplex subeenheid 1

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200, Immunoprecipitation: 1:50, Flow Cytometry: 1:50

O14561

Human, Mouse, Rat

A synthesized peptide derived from human NDUFAB1 was used as the immunogen for the NDUFAB1 antibody.

30N94

WB, IHC, IP, FACS

Affinity-chromatography

Liquid

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

This NDUFAB1 antibody is available for research use only.

Store the NDUFAB1 antibody at -20°C.

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Optimal dilution of the NDUFAB1 antibody should be determined by the researcher.

Cytoplasm (Mitochondria)

Immunohistochemical staining of NDUFAB1 using anti-NDUFAB1 antibody. NDUFAB1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NDUFAB1 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.