Nefrineantilichaam

Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth (1) . Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm (2,3) .

4648

O60500

NP_004637

Human, Mouse

7979

Unconjugated

Primary Antibodies

Induced Expression Validation (Figure 6) : Nephrin expression detected by anit-Nephrin antibodies was up-regulated by curcumin treatment in type 1 diabetic rats. Induced Expression Validation Localization Validation (Figure 7) : Nephrin detected by anti-Nephrin antibodies is colocalized with PKGIα at the tips of the cell processes in rat podocytes. Localization Validation Regulated expression validation (Figure 10) : Nephrin expression detected by anit-Nephrin antibodies was down-regulated by PEGF treatment (NGP or HGP), which was reversed by the addition of C3 transferase in mouse podocyte cells. Regulated expression validation

Homeostasis

Nephrin antibody is affinity chromatography purified via peptide column.

Cat. No. 1303 - Human Brain Tissue Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Rat (93%) .

Nephrin antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

Nephrin antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

IgG

Human Nephrin has 2 isoforms, including isoform 1 (1241aa, 135kD) and isoform 2 (1201aa, 131kD) . Mouse Nephrin also has 2 isoforms, including isoform 1 (1256aa, 136kD) and isoform 2 (1242aa, 135kD) . Rat Nephrin has 3 isoforms, including isoform 1 (1252aa, 136kD), isoform 2 (1239aa, 135kD) and isoform 3 (1166aa, 127kD) . 7979 can detect human and mouse.

NPHS1

Cat. No. 1201 - HeLa Cell Lysate

Cat. No. 1224 - Daudi Cell Lysate

Cat. No. 1285 - C2C12 Cell Lysate

Nephrosis 1, congenital, Finnish type (nephrin)

Homo sapiens

Kestila et al. Mol. Cell 1998; 1:575-582.

Tryggvason. J. Am. Soc. Nephrol. 1999; 10:2440-5.

Tryggvason and Wartiovaara. Curr. Opin. Nephrol. Hypertens. 2001; 10:543-9.

24004644

22898567

23454089

22212473

30536192

21520075

29032153

NPHN, NPHS1, Renal glomerulus-specific cell adhesion receptor3

ELISA, WB, IHC-P, IF

4758822

Liquid