Nefrineantilichaam

This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.

Western blot: 1:100-1:200, Immunofluorescence: 1:10-1:50

O60500

Human

A portion of amino acids 1088-1117 from the human protein was used as the immunogen for this Nephrin antibody.

174CT2.1.1

WB, IF, ELISA

Ascites

Ascites

This Nephrin antibody is available for research use only.

Aliquot the Nephrin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

In ascites with 0.09% sodium azide

Titration of the Nephrin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Nephrin antibody western blot analysis in SK-BR-3 lysate. Ab used at 1:100 dilution. Predicted molecular weight ~150 kDa but often observed at a lower size.