Nesfatin

Nesfatin-1 is a metabolic polypeptide encoded in the N-terminal region of the precursor protein, Nucleobindin2 (NUCB2) . Originally identified as a hypothalamic neuropeptide, Nesfatin-1 is also expressed in other areas of the brain, and in pancreatic isletsβ-cells, gastric endocrine cells and adipocytes. Nesfatin-1 suppresses food intake and can regulate energy metabolism in a Leptin independent manner. Recombinant human Nesfatin-1 is a 9.7 kDa protein containing 82 amino acid residues.

Nesfatin, Nucleobindin 2 protein

4925

P80303

NP_005004

NM_005013.2

Human

Human

VPIDIDKTKV QNIHPVESAK IEPPDTGLYY DEYLKQVIDV LETDKHFREK LQKADIEEIK SGRLSKELDL VSHHVRTKLD EL

< 0.1 ng/µg of protein (< 1EU/µg)

> 98% by SDS-PAGE & HPLC analysis

Determined by an in vivo assay using healthy wild type male mice (C57BL/6J) . Mice were treated via intraperitoneal injection once at a dose of 4μg Nesfatin-1/gm of body weight. Significant effects on body weight and food consumption were observed relative to saline-treated controls.

82

Lyophilized

10mM Sodium Phosphate, pH 6.6

Water

9.7 kDa

E. coli