Neurale cel adhesiemolecuul L1 Antilichaam / NCAM-L1

L1 (L1 cell adhesion molecule) also known as CAML1, CD171, and HSAS is an adhesion molecule which plays an important role in the nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus) . This mouse monoclonal antibody recognizes the sequence immediately NH2-terminal to the tyrosine-based motif and binds L1 only when Y1176 is dephosphorylated. Phosphorylation of either T1172 or Y1176 strongly inhibits binding of 74-5H7 to the L1 cytoplasmic domain.

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

P32004

Human

A portion of amino acids 600-900 from human L1CAM protein was used as the immunogen for the Neural cell adhesion molecule L1 antibody.

L1CAM/13053R

IHC-P, WB

Protein A affinity

Purified

This Neural cell adhesion molecule L1 antibody is available for research use only.

Aliquot the Neural cell adhesion molecule L1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

1 mg/mL in 1X PBS; BSA free, sodium azide free

Optimal dilution of the Neural cell adhesion molecule L1 antibody should be determined by the researcher.

Cell surface, Cytoplasm

IHC staining of FFPE human brain tissue with Neural cell adhesion molecule L1 antibody (clone L1CAM/13053R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.