NF-ppaB p65 (pho Ser281) Antilichaam

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex.

5970

Q04206

NP_001138610.1

Human, Mouse, Rat

NF- kappaB p65 (phospho Ser281) antibody was raised against a peptide sequence around phosphorylation site of serine281 (E-L-S (p) -E-P) derived from Human NF- kappaB .

Unconjugated

Primary Antibodies

Phospho-Specific

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

1 mg/mL

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Phospho-Specific

Blue Ice

Store antibody at -20˚ C for up to one year.

65 kDa

IgG

The antibody detects endogenous level of NF- kappaB p65 only when phosphorylated at serine 281.

Western Blot: 1:500~1:1000

RELA

V-rel avian reticuloendotheliosis viral oncogene homolog A

Homo sapiens

Hochrainer K, Racchumi G, Anrather J (2013) J Biol Chem 288, 285-93. Dai Y, et al. (2008) Clin Cancer Res 14, 549-58. Seldon MP, et al. (2007) J Immunol 179, 7840-51.

NFKB3, Nuclear factor NF-kappa-B p65 subunit, RELA, TF65, p65

WB

223468681

Liquid