NFkB p65 (fosfo Thr254) Antilichaam

NF-κ -B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-κ -B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-κ -B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-κ -B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-κ -B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-κ -B complex which translocates to the nucleus. NF-κ -B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-κ -B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-κ -B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-κ -B complex.

5970

Q04206

NP_001138610.1

Human, Mouse, Rat

NFkB-p65 (Phospho-Thr254) antibody was raised against a peptide sequence around phosphorylation site of threonine 254 (F-R-T (p) -P-P) derived from Human NFκ B-p65.

Unconjugated

Primary Antibodies

Phospho-Specific, Apoptosis, Cancer

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

1 mg/mL

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Phospho-Specific

Blue Ice

Store antibody at -20˚ C for up to one year.

65 kDa

This antibody detects endogenous level of NFκ B p65 only when phosphorylated at threonine 254.

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

RELA

V-rel avian reticuloendotheliosis viral oncogene homolog A

Homo sapiens

Yeh PY, et al. (2004) J Biol Chem. 279 (25) : 26143-26148. Ryo A, et al. (2003) Mol Cell. 12 (6) : 1413-1426. Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179. Baeuerle P A, et al. (1996) Cell 87:13-20. Haskill S, et al. (1991) Cell 65:1281-1289.

P65, NFKB3, CTNNB, OK/SW-cl.35, PRO2286, RELA, TF65, Transcription factor p65, p65

WB, IHC, IF

223468681

Liquid