Niet-gecoat cAMP (Cyclische adenosinemonofosfaat) ELISA Kit

CAMP

This kit recognizes cAMP in samples.No significant cross-reactivity or interference between cAMP and analogues was observed

3'-5'-cyclic adenosine monophosphate; CAMP; Cyclic AMP; Cyclic adenosine monophosphate

Competitive-ELISA

ELISA

Signal Transduction

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with cAMP. During the reaction, cAMP in the sample or standard competes with a fixed amount of cAMP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to cAMP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of cAMP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

24 h

Serum, plasma and other biological fluids

1.56-100 ng/mL

Both intra-CV and inter-CV are < 10%

80%-120%

Ice bag

-20°C,12 months

https://789.bio/eb/rNf3mgM0

Universal

This ELISA kit applies to the in vitro quantitative determination of cAMP concentrations in serum, plasma and other biological fluids.