Niet-gecoat Mouse Dkk3 (Dickkopf-gerelateerd eiwit 3) ELISA Kit

Dkk3

Q9QUN9

This kit recognizes Mouse Dkk3 in samples.No significant cross-reactivity or interference between Mouse Dkk3 and analogues was observed

DKK3; Dickkopf-3; Dickkopf-Related Protein 3; Dkk-3; REIC; hDkk-3

Sandwich-ELISA

ELISA

Signal Transduction; Stem cells

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Dkk3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Dkk3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Dkk3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Dkk3. You can calculate the concentration of Human Dkk3 in the samples by comparing the OD of the samples to the standard curve.

24 h

Serum, plasma and other biological fluids

0.16-10 ng/mL

Both intra-CV and inter-CV are < 10%

80%-120%

Ice bag

-20°C,12 months

https://789.bio/eb/kv8SV4Mx

Mouse

This ELISA kit applies to the in vitro quantitative determination of Mouse Dkk3 concentrations in serum, plasma and other biological fluids.