Niet-gecoat Mouse αFP (Alpha-Fetoproteïne) ELISA Kit

ΑFP

P02772

This kit recognizes Mouse αFP in samples.No significant cross-reactivity or interference between Mouse αFP and analogues was observed

AFP; FETA; HPAFP; Alpha-fetoglobulin; oralpha fetal protein

Sandwich-ELISA

ELISA

Cancer; Cardiovascular; Neuroscience; CellBiology; Developmental Biology

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human αFP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human αFP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human αFP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human αFP. You can calculate the concentration of Human αFP in the samples by comparing the OD of the samples to the standard curve.

24 h

Serum, plasma and other biological fluids

0.16-10 ng/mL

Both intra-CV and inter-CV are < 10%

80%-120%

Ice bag

-20°C,12 months

https://789.bio/eb/yjy5mozy

Mouse

This ELISA kit applies to the in vitro quantitative determination of Mouse αFP concentrations in serum, plasma and other biological fluids.