Niet-gecoat Mouse GFAP (gliaal fibrillair zuur eiwit) ELISA Kit

GFAP

P03995

This kit recognizes Mouse GFAP in samples.No significant cross-reactivity or interference between Mouse GFAP and analogues was observed

GFAP; Glial Fibrillary Acidic Protein

Sandwich-ELISA

ELISA

Cancer; Neuroscience; Signal Transduction; Stem cells; Tags and CellMarkers

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GFAP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GFAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GFAP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GFAP. You can calculate the concentration of Human GFAP in the samples by comparing the OD of the samples to the standard curve.

24 h

Serum, plasma and other biological fluids

7.81-500 pg/mL

Both intra-CV and inter-CV are < 10%

80%-120%

Ice bag

-20°C,12 months

https://789.bio/eb/4Vqgz9mg

Mouse

This ELISA kit applies to the in vitro quantitative determination of Mouse GFAP concentrations in serum, plasma and other biological fluids.