Niet-gecoat Mouse SCFR (Stemcel Groei Factor Receptor) ELISA Kit

SCFR

P05532

This kit recognizes Mouse SCFR in samples.No significant cross-reactivity or interference between Mouse SCFR and analogues was observed

Proto-oncogene c-Kit; tyrosine-protein kinase Kit; CD117; KIT; PBT

Sandwich-ELISA

ELISA

Immunology; Stem Cells

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SCFR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SCFR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SCFR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SCFR. You can calculate the concentration of Human SCFR in the samples by comparing the OD of the samples to the standard curve.

24 h

Serum, plasma and other biological fluids

1.56-100 ng/mL

Both intra-CV and inter-CV are < 10%

80%-120%

Ice bag

-20°C,12 months

https://789.bio/eb/ngteWRpq

Mouse

This ELISA kit applies to the in vitro quantitative determination of Mouse SCFR concentrations in serum, plasma and other biological fluids.