Niet-gecoat Mouse TNFSF8 (tumornecrosefactor ligand superfamilielid 8) ELISA Kit

TNFSF8

P32972

This kit recognizes Mouse TNFSF8 in samples.No significant cross-reactivity or interference between Mouse TNFSF8 and analogues was observed

TNFSF; Cd30l; Cd30lg; Tnfsf8; TNFSF8; CD153; CD30 ligand; Tumor necrosis factor ligand superfamily member 8

Sandwich-ELISA

ELISA

Immunology

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TNFSF8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNFSF8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNFSF8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TNFSF8. You can calculate the concentration of Human TNFSF8 in the samples by comparing the OD of the samples to the standard curve.

24 h

Serum, plasma and other biological fluids

0.31-20 ng/mL

Both intra-CV and inter-CV are < 10%

80%-120%

Ice bag

-20°C,12 months

https://789.bio/eb/kr2UKJ0M

Mouse

This ELISA kit applies to the in vitro quantitative determination of Mouse TNFSF8 concentrations in serum, plasma and other biological fluids.