Niet-gecoat Rat ACTH (adrenocorticotroop hormoon) ELISA Kit

ACTH

P01189

This kit recognizes Rat ACTH in samples.No significant cross-reactivity or interference between Rat ACTH and analogues was observed

Pomc1; Pomc

Competitive-ELISA

ELISA

Cancer; Metabolism; Neuroscience; Signal Transduction

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat ACTH. During the reaction, Rat ACTH in the sample or standard competes with a fixed amount of Rat ACTH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat ACTH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat ACTH in tested samples can be calculated by comparing the OD of the samples to the standard curve.

24 h

Serum, plasma and other biological fluids

15.63-1000 pg/mL

Both intra-CV and inter-CV are < 10%

80%-120%

Ice bag

-20°C,12 months

https://789.bio/eb/8nNhwMyJ

Rat

This ELISA kit applies to the in vitro quantitative determination of Rat ACTH concentrations in serum, plasma and other biological fluids.