Niet-gecoat rat-TFPI (Tissue Factor Pathway Inhibitor) ELISA Kit

TFPI

Q02445

This kit recognizes Rat TFPI in samples.No significant cross-reactivity or interference between Rat TFPI and analogues was observed

TFPI; EPI; LACI; TFI; TFPI1

Sandwich-ELISA

ELISA

Cancer; Cardiovascular

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TFPI. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TFPI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TFPI, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TFPI. You can calculate the concentration of Human TFPI in the samples by comparing the OD of the samples to the standard curve.

24 h

Serum, plasma and other biological fluids

78.13-5000 pg/mL

Both intra-CV and inter-CV are < 10%

80%-120%

Ice bag

-20°C,12 months

https://789.bio/eb/WVks6SxQ

Rat

This ELISA kit applies to the in vitro quantitative determination of Rat TFPI concentrations in serum, plasma and other biological fluids.