NIH/3T3 Lysaat

NIH/3T3 cell line was established from NIH Swiss mouse embryo cultures in the same manner as the original random bred 3T3 and the inbred BALB/c 3T3. These cells are highly contact inhibited and are sensitive to sarcoma virus focus formation and leukaemia virus propagation. Cells have now lost their contact inhibition.

Mouse

WB

2 mg/mL (determined by BCA assay)

NIHT3, ATGL0017-10 µg, ATGL0017-20 µg, ATGL0017-50 µg, ATGL0017-100 µg, ATGL0017-250 µg, ATGL0017-500 µg, ATGL0017-1 mg, ATGL0017-10, ATGL0017-20, ATGL0017-50, ATGL0017-100, ATGL0017-250, ATGL0017-500, ATGL0017-1

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Lysates

Mouse

All lanes : NIH/3T3 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196) ;

Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

Western Blot analyzed by both GAPDH and Beta tubulin antibody