Nivolumab ELISA Kit

PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant human PD1 antigen has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Nivolumab in the sample competitively binds to the pre-coated protein with biotin-labeled Nivolumab. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Nivolumab bound in the initial step. The color development is stopped and the intensity of the color is measured.

BMS-936558, MDX-1106, ONO-4538, CAS: 946414-94-4

Used for the quantitative determination of Nivolumab concentration in serum, plasma and cell culture supernatant.

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.

2-8 °C

For Research Use Only.