NKX2.1 Antilichaam / TFF-1 / Schildklier transcriptiefactor 1

Recognizes a protein of 40kDa, identified as Thyroid transcription factor-1 (TTF-1) and Homeobox protein Nkx-2.1. NKX2.1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-NKX2.1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. Loss of NKX2.1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. NKX2.1 reactivity is also seen in thyroid malignancies.

Immunohistochemistry (FFPE) : 1-2 µg/mL

P43699

Human

A portion of amino acids 1-200 from human TTF-1 protein was used as the immunogen for the NKX2.1 antibody.

NX2.1/9033

IHC-P

Protein G affinity

Purified

This NKX2.1 antibody is available for research use only.

Aliquot the NKX2.1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the NKX2.1 antibody should be determined by the researcher.

Nucleus

IHC staining of FFPE human thyroid tissue with NKX2.1 antibody (clone NX2.1/9033) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.