NMDA NR2B (fosfo Tyr1472) Antilichaam

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) . The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death. Overexpression of the NR2B subunit of the receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression. Tyr1472 on NR2B is phosphorylated and this may lead to the increased expression of the NMDAR at the synapse that plays a role in synaptic plasticity.

24410

Q00960

Q00960

Human, Mouse, Rat

NMDA NR2B (Tyr1472) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphorylated Tyr1472 of rat NMDA NR2B.

Unconjugated

Primary Antibodies

Neuroscience, Phospho-Specific

Affinity Purified

Batch dependent

Phospho-Specific

Blue Ice

For long term storage –80˚ C is recommended, but shorter term storage at –20˚ C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

180

NMDA antibody is specific for ~180 kD NMDAR NR2B phosphorylated at Tyr1472.

The labeling of the NR2B protein in Western blots of rat brain is blocked by the phosphopeptide but not by the dephosphopeptide. The antibody also labels bands at 65k and 115k. Applications include Dot Blots (DB) and Western Blots (WB) . Human, mouse, and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

Grin2b

Glutamate receptor, ionotropic, N-methyl D-aspartate 2B

Rattus norvegicus

Loftis, J.M. and Janowsky, A., "The N-methyl-D-aspartate receptor subunit NR2B: localization, functional properties, regulation, and clinical implications, " Pharmacol. Ther., 97 (2003) 55 - 85.

Clayton, D.A., Mesches, M.H., Alvarez, E., Bickford, P.C., and Browning, M.D., "A hippocampal NR2B deficit can mimic age-related changes in long-term potentiation and spatial learning in the Fischer 344 rat, " J. Neurosci., 22 (2002) 3628 - 3637.

Grosshans, D.R., Clayton, D.A., Coultrap, S.J., and Browning, M.D., "LTP leads to rapid surface expression on NMDA not AMPA receptors in adult rat CA1, " Nature Neurosci., 5 (2002) 27 - 33.

Snell, L.D., Nunley, K.R., Lickteig, R.L., Browning, M.D., Tabakoff, B., and Hoffman, P.L., "Regional and subunit specific changes in NMDA receptor mRNA and immunoreactivity in mouse brain following chronic ethanol ingestion, " Mol. Brain Res., 40 (1996) 71 - 78.

WB

548372

Liquid