NME2 cDNA

NME2, also known as NM23B, is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. NME1 and NME2 comprise the 152 amino acid A and B polypeptide chains of the NM23 enzyme, respectively. NME2 is identical to the beta subunit of human erythrocyte NDP kinase. NDP kinases are involved in the synthesis of nucleoside triphosphates, and NM23 may act in the regulation of signal transduction by complexing with G proteins, causing activation/inactivation of developmental pathways

NDKB, NDPK-B, NDPKB, NM23-H2, NM23B, PUF

17q21.3

Human

PATGen (puc19-derived cloning vector)

ATGGCCAACC TGGAGCGCAC CTTCATCGCC ATCAAGCCGG ACGGCGTGCA GCGCGGCCTG GTGGGCGAGA TCATCAAGCG CTTCGAGCAG AAGGGATTCC GCCTCGTGGC CATGAAGTTC CTCCGGGCCT CTGAAGAACA CCTGAAGCAG CACTACATTG ACCTGAAAGA CCGACCATTC TTCCCTGGGC TGGTGAAGTA CATGAACTCA GGGCCGGTTG TGGCCATGGT CTGGGAGGGG CTGAACGTGG TGAAGACAGG CCGAGTGATG CTTGGGGAGA CCAATCCAGC AGATTCAAAG CCAGGCACCA TTCGTGGGGA CTTCTGCATT CAGGTTGGCA GGAACATCAT TCATGGCAGT GATTCAGTAA AAAGTGCTGA AAAAGAAATC AGCCTATGGT TTAAGCCTGA AGAACTGGTT GACTACAAGT CTTGTGCTCA TGACTGGGTC TATGAATAA

NME2, NDKB, NDPK-B, NDPKB, NM23-H2, NM23B, PUF, NME2, ATGD0013-10 µg, ATGD0013-20 µg, ATGD0013-50 µg, ATGD0013-100 µg, ATGD0013-250 µg, ATGD0013-500 µg, ATGD0013-1 mg, ATGD0013-10, ATGD0013-20, ATGD0013-50, ATGD0013-100, ATGD0013-250, ATGD0013-500, ATGD0013-1

Store the plasmid at -20C.

Lyophilized

Metabolism

156491

NP_001018149.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_001018139.2

459bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.